کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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3424413 | 1227220 | 2012 | 10 صفحه PDF | دانلود رایگان |
Porcine reproductive and respiratory syndrome virus (PRRSV) is known to interfere with the signaling of type I interferons (IFNs). Here we found PRRSV A2MC2 induced type I IFNs in cultured cells. A2MC2 replication in MARC-145 cells resulted in the synthesis of IFN-α2 protein, transcript elevation of the IFN-stimulated genes ISG15 and ISG56, and the proteins of the signal transducer and activator of transcription 2 (STAT2) and ISG56. A2MC2 infection of primary porcine pulmonary alveolar macrophages (PAMs) also led to the elevation of the two proteins, but had little cytopathic effect. Furthermore, A2MC2 infection of MARC-145 or PAM cells had no detectable inhibitory effect on the ability of IFN-α to induce an antiviral response. Sequencing analysis indicated that A2MC2 was closely related to VR-2332 and Ingelvac PRRS MLV with an identity of 99.8% at the nucleotide level. The identification of this IFN-inducing PRRSV isolate may be beneficial for vaccine development against PRRS.
► We discovered a PRRSV isolate A2MC2 that induces synthesis of type I interferons.
► A2MC2 induces IFN production in both MARC-145 cells and PAMs.
► A2MC2 is a Type 2 PRRSV closely related to VR-2332 and MLV strains.
► Like MLV strain, A2MC2 infection of PAMs leads to undetectable adverse effect.
Journal: Virology - Volume 432, Issue 2, 25 October 2012, Pages 261–270