کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3424440 1227220 2012 16 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A novel coding-region RNA element modulates infectious dengue virus particle production in both mammalian and mosquito cells and regulates viral replication in Aedes aegypti mosquitoes
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
A novel coding-region RNA element modulates infectious dengue virus particle production in both mammalian and mosquito cells and regulates viral replication in Aedes aegypti mosquitoes
چکیده انگلیسی

Dengue virus (DENV) is an enveloped flavivirus with a positive-sense RNA genome transmitted by Aedes mosquitoes, causing the most important arthropod-borne viral disease affecting humans. Relatively few cis-acting RNA regulatory elements have been described in the DENV coding-region. Here, by introducing silent mutations into a DENV-2 infectious clone, we identify the conserved capsid-coding region 1 (CCR1), an RNA sequence element that regulates viral replication in mammalian cells and to a greater extent in Ae. albopictus mosquito cells. These defects were confirmed in vivo, resulting in decreased replication in Ae. aegypti mosquito bodies and dissemination to the salivary glands. Furthermore, CCR1 does not regulate translation, RNA synthesis or virion retention but likely modulates assembly, as mutations resulted in the release of non-infectious viral particles from both cell types. Understanding the role of CCR1 could help characterize the poorly-defined stage of assembly in the DENV life cycle and uncover novel anti-viral targets.


► CCR1 is conserved in nucleotide sequence and predicted structure in DENV and TBE.
► CCR1 is a regulatory RNA sequence element in mosquito and less so in mammalian cells.
► Mutating CCR1 reduces viral replication & dissemination in Ae. aegypti mosquitoes.
► CCR1 does not affect viral RNA translation, synthesis, stability or virus retention.
► Mutating CCR1 results in the release of non-infectious viral particles.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 432, Issue 2, 25 October 2012, Pages 511–526
نویسندگان
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