کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3424490 | 1227225 | 2012 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Human papillomavirus type 16 E6 and E 7 proteins alter NF-kB in cultured cervical epithelial cells and inhibition of NF-kB promotes cell growth and immortalization Human papillomavirus type 16 E6 and E 7 proteins alter NF-kB in cultured cervical epithelial cells and inhibition of NF-kB promotes cell growth and immortalization](/preview/png/3424490.png)
The NF-kB family of transcription factors regulates important biological functions including cell growth, survival and the immune response. We found that Human Papillomavirus type 16 (HPV-16) E7 and E6/E7 proteins inhibited basal and TNF-alpha-inducible NF-kB activity in human epithelial cells cultured from the cervical transformation zone, the anatomic region where most cervical cancers develop. In contrast, HPV-16 E6 regulated NF-kB in a cell type- and cell growth-dependent manner. NF-kB influenced immortalization of cervical cells by HPV16. Inhibition of NF-kB by an IkB alpha repressor mutant increased colony formation and immortalization by HPV-16. In contrast, activation of NF-kB by constitutive expression of p65 inhibited proliferation and immortalization. Our results suggest that inhibition of NF-kB by HPV-16 E6/E7 contributes to immortalization of cells from the cervical transformation zone.
► We examined the effect on HPV-16 E6 and E7 on NF-kB activity.
► E7 inhibited NF-kB.
► E6 altered NF-kB in a cell type and cell growth dependent manner.
► Inhibition of NF-kB increased immortalization by HPV-16 E6/E7.
► Activation of NF-kB decreased immortalization.
Journal: Virology - Volume 425, Issue 1, 30 March 2012, Pages 53–60