کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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3424603 | 1227235 | 2011 | 8 صفحه PDF | دانلود رایگان |

Our previous study has shown that HIV-1 replicated at higher levels in neonatal (cord) blood monocytes/macrophages and T-lymphocytes compared with adult blood cells. However, it is not known whether this differential HIV-1 replication also occurs in naive and/or memory T-lymphocytes. We, therefore, compared HIV-1 replication in CD3+ and CD4+ naive (CD45RA+) and memory (CD45RO+) T-lymphocytes isolated from five cord and adult blood donors. We found that HIV-1 replicated at higher levels in both CD3+ and CD4+ CD45RA+ and CD45RO+ T-lymphocytes isolated from cord blood compared with adult blood. In addition, there was no difference in the cell surface expression of CD4, CXCR4 and CCR5 on cord blood CD45RA+ and CD45RO+ T-lymphocytes compared with adult blood cells. Furthermore, we found that there was an increase in HIV-1 gene expression in cord blood CD45RA+ and CD45RO+ T-lymphocytes compared with adult blood cells by using a single-cycle replication competent HIV-1-NL4-3-Env−R+ luciferase amphotropic virus, which measures HIV-1 transcriptional activity independent of CD4 and CXCR4 or CCR5 expression. In summary, HIV-1 replicated at higher levels in cord blood CD45RA+ and CD45RO+ T-lymphocytes compared with adult blood cells and this differential replication is influenced at the level of HIV-1 gene expression.
Journal: Virology - Volume 413, Issue 1, 25 April 2011, Pages 39–46