کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3424603 1227235 2011 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
HIV-1 replication and gene expression occur at higher levels in neonatal blood naive and memory T-lymphocytes compared with adult blood cells
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
HIV-1 replication and gene expression occur at higher levels in neonatal blood naive and memory T-lymphocytes compared with adult blood cells
چکیده انگلیسی

Our previous study has shown that HIV-1 replicated at higher levels in neonatal (cord) blood monocytes/macrophages and T-lymphocytes compared with adult blood cells. However, it is not known whether this differential HIV-1 replication also occurs in naive and/or memory T-lymphocytes. We, therefore, compared HIV-1 replication in CD3+ and CD4+ naive (CD45RA+) and memory (CD45RO+) T-lymphocytes isolated from five cord and adult blood donors. We found that HIV-1 replicated at higher levels in both CD3+ and CD4+ CD45RA+ and CD45RO+ T-lymphocytes isolated from cord blood compared with adult blood. In addition, there was no difference in the cell surface expression of CD4, CXCR4 and CCR5 on cord blood CD45RA+ and CD45RO+ T-lymphocytes compared with adult blood cells. Furthermore, we found that there was an increase in HIV-1 gene expression in cord blood CD45RA+ and CD45RO+ T-lymphocytes compared with adult blood cells by using a single-cycle replication competent HIV-1-NL4-3-Env−R+ luciferase amphotropic virus, which measures HIV-1 transcriptional activity independent of CD4 and CXCR4 or CCR5 expression. In summary, HIV-1 replicated at higher levels in cord blood CD45RA+ and CD45RO+ T-lymphocytes compared with adult blood cells and this differential replication is influenced at the level of HIV-1 gene expression.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 413, Issue 1, 25 April 2011, Pages 39–46
نویسندگان
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