کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3424638 | 1227236 | 2011 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Use of a tandem affinity purification assay to detect interactions between West Nile and dengue viral proteins and proteins of the mosquito vector Use of a tandem affinity purification assay to detect interactions between West Nile and dengue viral proteins and proteins of the mosquito vector](/preview/png/3424638.png)
West Nile and dengue viruses are (re)emerging mosquito-borne flaviviruses that cause significant morbidity and mortality in man. The identification of mosquito proteins that associate with flaviviruses may provide novel targets to inhibit infection of the vector or block transmission to humans. Here, a tandem affinity purification (TAP) assay was used to identify 18 mosquito proteins that interact with dengue and West Nile capsid, envelope, NS2A or NS2B proteins. We further analyzed the interaction of mosquito cadherin with dengue and West Nile virus envelope protein using co-immunoprecipitation and immunofluorescence. Blocking the function of select mosquito factors, including actin, myosin, PI3-kinase and myosin light chain kinase, reduced both dengue and West Nile virus infection in mosquito cells. We show that the TAP method may be used in insect cells to accurately identify flaviviral–host protein interactions. Our data also provides several targets for interrupting flavivirus infection in mosquito vectors.
► TAP assay can be used to identify flaviviral–arthropod protein interactions.
► Mosquito proteins that bind flaviviral proteins are important for infection.
► Inhibiting actin, myosin and PI3-kinase reduces both West Nile and dengue infection in mosquito cells.
Journal: Virology - Volume 417, Issue 1, 15 August 2011, Pages 179–187