کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3424679 | 1227238 | 2012 | 10 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Emergence of novel equine arteritis virus (EAV) variants during persistent infection in the stallion: Origin of the 2007 French EAV outbreak was linked to an EAV strain present in the semen of a persistently infected carrier stallion Emergence of novel equine arteritis virus (EAV) variants during persistent infection in the stallion: Origin of the 2007 French EAV outbreak was linked to an EAV strain present in the semen of a persistently infected carrier stallion](/preview/png/3424679.png)
During the summer of 2007, an outbreak of equine viral arteritis (EVA) occurred in Normandy (France). After investigation, a link was suggested between an EAV carrier stallion (A) and the index premise of the outbreak. The full-length nucleotide sequence analysis of a study reference strain (F27) isolated from the lung of a foal revealed a 12,710 nucleotides EAV genome with unique molecular hallmarks in the 5′UTR leader sequence and the ORF1a sequence encoding the non-structural protein 2. The evolution of the viral population in the persistently infected Stallion A was then studied by cloning ORFs 3 and 5 of the EAV genome from four sequential semen samples which were collected between 2000 and 2007. Molecular analysis of the clones confirmed the likely implication of Stallion A in the origin of this outbreak through the yearly emergence of new variants genetically similar to the F27 strain.
Journal: Virology - Volume 423, Issue 2, 20 February 2012, Pages 165–174