کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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3424693 | 1594265 | 2011 | 11 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: The HIV-1 Nef protein has a dual role in T cell receptor signaling in infected CD4+ T lymphocytes The HIV-1 Nef protein has a dual role in T cell receptor signaling in infected CD4+ T lymphocytes](/preview/png/3424693.png)
The phenotypic changes that are induced by immune activation in CD4+ T lymphocytes provide an optimal environment for efficient HIV-1 replication in these cells. The pathogenic Nef protein of HIV-1 modulates the T cell receptor (TCR) signaling, but whether this has a positive or negative effect on cellular activation is a matter of debate. Here we have investigated the response to TCR stimulation of primary CD4+ T lymphocytes infected with wt or Nef-deficient HIV-1. Results show that, in freshly isolated quiescent T cells, Nef superinduces NFAT and IL-2 production bypassing early TCR effector molecules. Conversely, the early phosphorylation of PLC-γ1, the induction of NFAT, and the expression of IL-2 are impaired by Nef in sub-optimally activated/resting T cells. Our data indicate that Nef has a dual role in the modulation of TCR signaling aimed at favoring HIV-1 replication and spread in both quiescent and metabolically active CD4+ T lymphocytes.
Journal: Virology - Volume 410, Issue 2, 20 February 2011, Pages 316–326