کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3424698 | 1594265 | 2011 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Baculovirus resistance in codling moth (Cydia pomonella L.) caused by early block of virus replication Baculovirus resistance in codling moth (Cydia pomonella L.) caused by early block of virus replication](/preview/png/3424698.png)
An up to 10,000-fold resistance against the biocontrol agent Cydia pomonella granulovirus (CpGV) was observed in field populations of codling moth, C. pomonella, in Europe. Following different experimental approaches, a modified peritrophic membrane, a modified midgut receptor, or a change of the innate immune response could be excluded as possible resistance mechanisms. When CpGV replication was traced by quantitative PCR in different tissues of susceptible and resistant insects after oral and intra-hemocoelic infection, no virus replication could be detected in any of the tissues of resistant insects, suggesting a systemic block prior to viral DNA replication. This conclusion was corroborated by fluorescence microscopy using a modified CpGV (bacCpGVhsp-eGFP) carrying enhanced green fluorescent gene (eGFP), which showed that infection in resistant insects did not spread. In conclusion, the different lines of evidence indicate that CpGV can enter but not replicate in the cells of resistant codling moth larvae.
Journal: Virology - Volume 410, Issue 2, 20 February 2011, Pages 360–367