کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3425102 1227269 2010 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
DNA crunching by a viral packaging motor: Compression of a procapsid-portal stalled Y-DNA substrate
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
DNA crunching by a viral packaging motor: Compression of a procapsid-portal stalled Y-DNA substrate
چکیده انگلیسی

Many large double-stranded DNA viruses employ high force-generating ATP-driven molecular motors to package to high density their genomes into empty procapsids. Bacteriophage T4 DNA translocation is driven by a two-component motor consisting of the procapsid portal docked with a packaging terminase-ATPase. Fluorescence resonance energy transfer and fluorescence correlation spectroscopic (FRET-FCS) studies of a branched (Y-junction) DNA substrate with a procapsid-anchoring leader segment and a single dye molecule situated at the junction point reveal that the “Y-DNA” stalls in proximity to the procapsid portal fused to GFP. Comparable structure Y-DNA substrates containing energy transfer dye pairs in the Y-stem separated by 10 or 14 base pairs reveal that B-form DNA is locally compressed 22–24% by the linear force of the packaging motor. Torsional compression of duplex DNA is thus implicated in the mechanism of DNA translocation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 398, Issue 2, 15 March 2010, Pages 224–232
نویسندگان
, , , ,