کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3425139 1227271 2010 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
RNA induced polymerization of the Borna disease virus nucleoprotein
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
RNA induced polymerization of the Borna disease virus nucleoprotein
چکیده انگلیسی

The Borna disease virus (BDV) nucleoprotein (N) monomer resembles the nucleoprotein structures from rabies virus (RABV) and vesicular stomatitis virus (VSV). We show that BDV N assembles into ring- and string-like structures in the presence of 5′ genomic BDV RNA. RNA induced polymerization is partly RNA-specific since polymerization is inefficient in the presence of 3′ genomic BDV RNA or E. coli RNA. Mutagenesis of basic residues located in the cleft made up by the N- and C-terminal domains of N abrogate RNA-induced polymerization indicating that BDV N binds RNA similarly as observed in case of RABV and VSV N–RNA complexes. Bound RNA is not protected and sensitive to degradation. N–RNA polymers form complexes with the phosphoprotein P as required for functional transcription or replication units. Our data indicate that BDV N utilizes similar structural principles for N–RNA and N–P–RNA complex formation as observed for related negative strand RNA viruses.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 397, Issue 1, 5 February 2010, Pages 64–72
نویسندگان
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