کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3425332 1227278 2009 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Expression of the highly conserved vaccinia virus E6 protein is required for virion morphogenesis
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Expression of the highly conserved vaccinia virus E6 protein is required for virion morphogenesis
چکیده انگلیسی

The vaccinia virus E6R gene (VACVWR062) is conserved in all members of the poxvirus family and encodes a protein associated with the mature virion. We confirmed this association and provided evidence for an internal location. An inducible mutant that conditionally expresses E6 was constructed. In the absence of inducer, plaque formation and virus production were severely inhibited in several cell lines, whereas some replication occurred in others. This difference could be due to variation in the stringency of repression, since we could not isolate a stable deletion mutant even in the more “permissive” cells. Under non-permissive conditions, viral late proteins were synthesized but processing of core proteins was inefficient, indicative of an assembly block. Transmission electron microscopy of sections of cells infected with the mutant in the absence of inducer revealed morphogenetic defects with crescents and empty immature virions adjacent to dense inclusions of viroplasm. Mature virions were infrequent and cores appeared to have lucent centers.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 386, Issue 2, 10 April 2009, Pages 478–485
نویسندگان
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