کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3425445 | 1227285 | 2009 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification of a lipid kinase as a host factor involved in hepatitis C virus RNA replication
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Identification of a lipid kinase as a host factor involved in hepatitis C virus RNA replication Identification of a lipid kinase as a host factor involved in hepatitis C virus RNA replication](/preview/png/3425445.png)
چکیده انگلیسی
A functional screen of an adenovirus-delivered shRNA library that targets ∼ 4500 host genes was performed to identify cellular factors that regulate hepatitis C virus (HCV) sub-genomic RNA replication. Seventy-three hits were further examined by siRNA oligonucleotide-directed knockdown, and silencing of the PI4KA gene was demonstrated to have a significant effect on the replication of a HCV genotype 1b replicon. Using transient siRNA oligonucleotide transfections and stable shRNA knockdown clones in HuH-7 cells, the PI4KA gene was shown to be essential for the replication of all HCV genotypes tested (1a, 1b and 2a) but not required for bovine viral diarrhea virus (BVDV) RNA replication.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 387, Issue 1, 25 April 2009, Pages 5–10
Journal: Virology - Volume 387, Issue 1, 25 April 2009, Pages 5–10
نویسندگان
Frédéric H. Vaillancourt, Louise Pilote, Mireille Cartier, Julie Lippens, Michel Liuzzi, Richard C. Bethell, Michael G. Cordingley, George Kukolj,