کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3425516 | 1227288 | 2009 | 12 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Host-dependent roles of the viral 5′ untranslated region (UTR) in RNA stabilization and cap-independent translational enhancement mediated by the 3′ UTR of Red clover necrotic mosaic virus RNA1 Host-dependent roles of the viral 5′ untranslated region (UTR) in RNA stabilization and cap-independent translational enhancement mediated by the 3′ UTR of Red clover necrotic mosaic virus RNA1](/preview/png/3425516.png)
The genome of Red clover necrotic mosaic virus (RCNMV) consists of RNA1 and RNA2, both lacking a cap structure and a poly(A)tail. RNA1 has a translational enhancer element (3′TE-DR1) in the 3′ untranslated region (UTR). In this study, we analyzed the roles of 5′ and 3′ UTRs of RNA1 in 3′TE-DR1-mediated cap-independent translation in cowpea and tobacco BY-2 protoplasts using a dual-luciferase (Luc) reporter assay system. Most mutations introduced into RNA1 5′ UTR in reporter Luc mRNA abolished or greatly reduced cap-independent translation in BY-2 protoplasts, whereas those mutations had no or much milder effects if any on translational activity in cowpea protoplasts. Our results suggest that a stem–loop structure predicted in the 5′ proximal region of RNA1 plays important roles in both translation and RNA stability. We also show that 3′TE-DR1-mediated cap-independent translation relies on a ribosome-scanning mechanism in both protoplasts.
Journal: Virology - Volume 391, Issue 1, 15 August 2009, Pages 107–118