کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3426362 1227327 2007 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Geminivirus pathogenicity protein C4 interacts with Arabidopsis thaliana shaggy-related protein kinase AtSKη, a component of the brassinosteroid signalling pathway
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Geminivirus pathogenicity protein C4 interacts with Arabidopsis thaliana shaggy-related protein kinase AtSKη, a component of the brassinosteroid signalling pathway
چکیده انگلیسی

Beet curly top virus (BCTV) C4 interacted with two members of the shaggy-related protein kinase family (AtSKη and AtSKζ) and a putative leucine-rich repeat receptor-like kinase (LRR-RLK) in a yeast two-hybrid assay. Tomato golden mosaic virus (TGMV) AC4 also bound with similar efficiency to AtSKη and AtSKζ but was unable to interact with the LRR-RLK. BCTV C4 interaction with AtSKη was confirmed using an in vitro binding assay. The protein kinases were capable of autophosphorylation in vitro and AtSKη phosphorylated BCTV C4 at threonine and serine residues. AtSKη phosphorylation of TGMV AC4 was significantly less efficient. The LRR-RLK did not efficiently phosphorylate BCTV C4. BCTV C4 localisation to the cell periphery in Nicotiana benthamiana was dependent on an intact N-terminal myristoylation motif, consistent with plasma membrane targeting. The intact motif was also required to produce the wild-type disease phenotype. Transient expression of BCTV C4 and TGMV AC4 derivatives in N. benthamiana identified additional amino acids within a central domain that contribute to the phenotype. The interaction with AtSKη indicates that BCTV C4 interacts with the brassinosteroid signalling pathway.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 362, Issue 2, 5 June 2007, Pages 428–440
نویسندگان
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