کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3426629 | 1227338 | 2006 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Enhancement of neutralizing activity of influenza virus-specific antibodies by serum components Enhancement of neutralizing activity of influenza virus-specific antibodies by serum components](/preview/png/3426629.png)
The role of serum components in enhancing virus neutralizing (VN) activity of influenza virus A/PR/8/34 hemagglutinin (HA)-specific MAbs in vitro was investigated. The degree of enhancement depended on the MAb's fine specificity and heavy chain isotype and on type of serum. Greatest enhancement (>100-fold) was seen with sera from immunodeficient mice that lacked serum immunoglobulin. At least two serum components were involved: C1q and a heat-resistant factor. C1q was mandatory for enhancement, and other components of the complement system were not required. C1q appeared to operate by improving MAb-mediated inhibition of virus attachment to host cells and was most effective with MAbs that inhibited virus attachment poorly on their own. The heat-resistant factor enhanced VN activity only in the presence of C1q and appeared to operate by enhancing VN activity at a post-attachment stage.
Journal: Virology - Volume 352, Issue 2, 1 September 2006, Pages 418–426