کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3427005 1227356 2006 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Rescue of internal scaffold-deleted Mason-Pfizer monkey virus particle production by plasma membrane targeting
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Rescue of internal scaffold-deleted Mason-Pfizer monkey virus particle production by plasma membrane targeting
چکیده انگلیسی

The Mason-Pfizer monkey virus (M-PMV) Gag protein follows a morphogenesis pathway in which immature capsids are preassembled within the cytoplasm before interaction with and budding through the plasma membrane. Intracytoplasmic assembly is facilitated by sequences within the p12 domain of Gag that we have termed the Internal Scaffold Domain (ISD). If M-PMV utilizes an ISD then what provides the equivalent function for most other retroviruses that assemble at the plasma membrane? To investigate the possibility that the membrane itself fulfills this role, we have combined functional deletion of the ISD with a mutation that disrupts intracellular targeting or with a plasma membrane targeting signal. By either modification, targeting of ISD-deleted Gag to the plasma membrane restores particle production. These results provide support for a model in which the plasma membrane and the D-type ISD provide an interchangeable scaffold-like function in retrovirus assembly.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 345, Issue 2, 20 February 2006, Pages 317–327
نویسندگان
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