کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3427016 1227356 2006 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification of DNA-binding sites for the activator involved in late transcription of the temperate lactococcal phage TP901-1
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Identification of DNA-binding sites for the activator involved in late transcription of the temperate lactococcal phage TP901-1
چکیده انگلیسی

Alt, encoded by the lactococcal phage TP901-1, is needed for late transcription. We identify Alt as a DNA-binding protein, and footprint analysis shows that Alt binds to a region containing four imperfect direct repeats (ALT boxes) located −76 to −32 relative to the Plate transcriptional start site. The importance of the ALT boxes was confirmed by deletion of one or two ALT boxes and by introducing mutations in ALT boxes 1 and 4. Alt is proposed to act as a tetramer or higher multimer activating transcription of TP901-1 late genes by binding to the four ALT boxes, and bending of the DNA may be important for transcriptional activation of Plate. Furthermore, our results suggest that DNA replication may be required for late transcription in TP901-1. Additionally, we identify gp28 of the related lactococcal phage Tuc2009 as an activator and show that the activators required for late transcription in TP901-1 and Tuc2009 are interchangeable.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 345, Issue 2, 20 February 2006, Pages 446–456
نویسندگان
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