کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3427022 1227356 2006 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Relationship between poliovirus negative-strand RNA synthesis and the length of the 3′ poly(A) tail
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Relationship between poliovirus negative-strand RNA synthesis and the length of the 3′ poly(A) tail
چکیده انگلیسی

The precise relationship between the length of the 3′ poly(A) tail and the replication and infectivity of poliovirus RNA was examined in this study. With both poly(A)11 and poly(A)12 RNAs, negative-strand synthesis was 1–3% of the level observed with poly(A)80 RNA. In contrast, increasing the length of the poly(A) tail from (A)12 to (A)13 resulted in about a ten-fold increase in negative-strand synthesis. This increase continued with each successive increase in poly(A) tail length. With poly(A)20 RNA, RNA synthesis approached the level observed with poly(A)80 RNA. A similar relationship was observed between poly(A) tail length and the infectivity of the viral RNA. A replication model is described which suggests that viral RNA replication is dependent on a poly(A) tail that is long enough to bind poly(A) binding protein and to act as a template for VPg uridylylation and negative-strand initiation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 345, Issue 2, 20 February 2006, Pages 509–519
نویسندگان
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