کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3427282 1227383 2007 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
In vitro assembly of a prohead-like structure of the Rhodobacter capsulatus gene transfer agent
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
In vitro assembly of a prohead-like structure of the Rhodobacter capsulatus gene transfer agent
چکیده انگلیسی

The gene transfer agent (GTA) is a phage-like particle capable of exchanging double-stranded DNA fragments between cells of the photosynthetic bacterium Rhodobacter capsulatus. Here we show that the major capsid protein of GTA, expressed in E. coli, can be assembled into prohead-like structures in the presence of calcium ions in vitro. Transmission electron microscopy (TEM) of uranyl acetate staining material and thin sections of glutaraldehyde-fixed material demonstrates that these associates have spherical structures with diameters in the range of 27–35 nm. The analysis of scanning TEM images revealed particles of mass ∼ 4.3 MDa, representing 101 ± 11 copies of the monomeric subunit. The establishment of this simple and rapid method to form prohead-like particles permits the GTA system to be used for genome manipulation within the photosynthetic bacterium, for specific targeted drug delivery, and for the construction of biologically based distributed autonomous sensors for environmental monitoring.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 364, Issue 1, 20 July 2007, Pages 95–102
نویسندگان
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