کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3427364 | 1227387 | 2007 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: A distinct single-stranded DNA-binding protein encoded by the Lactococcus lactis bacteriophage bIL67 A distinct single-stranded DNA-binding protein encoded by the Lactococcus lactis bacteriophage bIL67](/preview/png/3427364.png)
Single-stranded binding proteins (SSBs) are found to participate in various processes of DNA metabolism in all known organisms. We describe here a SSB protein encoded by the Lactococcus lactis phage bIL67 orf14 gene. It is the first noted attempt at characterizing a SSB protein from a lactococcal phage. The purified Orf14bIL67 binds unspecifically to ssDNA with the same high affinity as the canonical Bacillus subtilis SSB. Electrophoretic mobility-shift assays performed with mutagenized Orf14bIL67 protein derivatives suggest that ssDNA-binding occurs via a putative OB-fold structure predicted by three-dimensional modeling. The native Orf14bIL67 forms homotetramers as determined by gel filtration studies. These results allow distinguishing the first lactococcal phage protein with single-strand binding affinity, which defines a novel cluster of phage SSBs proteins. The possible role of Orf14bIL67 in phage multiplication cycle is also discussed.
Journal: Virology - Volume 363, Issue 1, 20 June 2007, Pages 104–112