کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3427402 1227390 2006 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Beilong virus, a novel paramyxovirus with the largest genome of non-segmented negative-stranded RNA viruses
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Beilong virus, a novel paramyxovirus with the largest genome of non-segmented negative-stranded RNA viruses
چکیده انگلیسی

During a subtraction study on gene expression in human kidney mesangial cells (HMCs), cDNA clones with sequence homology to paramyxovirus P, M and F genes were isolated. Subsequent investigation revealed that this particular HMC line was infected with a previously unknown paramyxovirus. Here, we report the isolation and genome characterization of this new virus, now named Beilong virus (BeV). The genome of BeV is 19,212 nucleotides (nt) in length and is the largest among all known members of the order Mononegavirales. The BeV genome contains eight genes in the order 3′-N-P/V/C-M-F-SH-TM-G-L-5′. The SH and TM genes code for a small hydrophobic protein of 76 aa and a transmembrane protein of 254 aa, respectively. The BeV G gene, at 4527 nt, codes for an attachment protein of 734 aa and contains two additional open reading frames (ORFs) in the 3′ half of the gene, coding for putative proteins of 299 and 394 aa in length. Although the exact origin of BeV is presently unknown, we provide evidence indicating that BeV was present in a rat mesangial cell line used in the same laboratory prior to the acquisition of the HMC line, suggesting a potential rodent origin for BeV.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 346, Issue 1, 1 March 2006, Pages 219–228
نویسندگان
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