کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3427484 1227399 2007 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Analysis of Vif-induced APOBEC3G degradation using an α-complementation assay
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Analysis of Vif-induced APOBEC3G degradation using an α-complementation assay
چکیده انگلیسی

Vif forms a complex with Elongin B/C, Cullin-5 and Rbx-1 to induce the polyubiquitination and proteasome-mediated degradation of human APOBEC3G (hA3G). These interactions serve as potential targets for anti-HIV-1 drug development. We have developed a cell culture-based assay to measure Vif-induced hA3G degradation. The assay is based on α-complementation, the ability of β-galactosidase fragments to complement in trans. hA3G expressed with a fused α-peptide was enzymatically active, complemented a coexpressed ω-fragment and could be targeted for degradation by Vif. Vif reduced β-galactosidase activity in the cell by 10–30-fold. The assay was validated by testing various hA3G and Vif point mutants. The assay accurately detected the effects of D128 in hA3G, and the BC box, Cul5 box and HCCH motifs of Vif. The results showed a strict association of Vif biological function with hA3G degradation. These findings support hA3G degradation as a requirement for Vif function. The Vif α-complementation assay may be a useful tool for the identification of Vif inhibitors.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 359, Issue 1, 1 March 2007, Pages 162–169
نویسندگان
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