کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3427564 1227417 2006 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Ikkepsilon regulates viral-induced interferon regulatory factor-3 activation via a redox-sensitive pathway
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Ikkepsilon regulates viral-induced interferon regulatory factor-3 activation via a redox-sensitive pathway
چکیده انگلیسی

Respiratory syncytial virus (RSV)-induced chemokine gene expression occurs through the activation of a subset of transcription factors, including Interferon Regulatory Factor (IRF)-3. In this study, we have investigated the signaling pathway leading to RSV-induced IRF-3 activation and whether it is mediated by intracellular reactive oxygen species (ROS) generation. Our results show that RSV infection induces expression and catalytic activity of IKKε, a noncanonical IKK-like kinase. Expression of a kinase-inactive IKKε blocks RSV-induced IRF-3 serine phosphorylation, nuclear translocation and DNA-binding, leading to inhibition of RANTES gene transcription, mRNA expression and protein synthesis. Treatment of alveolar epithelial cells with antioxidants or with NAD(P)H oxidase inhibitors abrogates RSV-induced chemokine secretion, IRF-3 phosphorylation and IKKε induction, indicating that ROS generation plays a fundamental role in the signaling pathway leading to IRF-3 activation, therefore, identifying a novel molecular target for the development of strategies aimed to modify the inflammatory response associated with RSV infection of the lung.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 353, Issue 1, 15 September 2006, Pages 155–165
نویسندگان
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