کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3427634 1227430 2006 19 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Characterization of Kaposi's sarcoma-associated herpesvirus (KSHV) K1 promoter activation by Rta
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Characterization of Kaposi's sarcoma-associated herpesvirus (KSHV) K1 promoter activation by Rta
چکیده انگلیسی

The K1 gene of Kaposi's sarcoma-associated herpesvirus (KSHV) encodes a 46-kDa transmembrane glycoprotein that possesses transforming properties, initiates signaling pathways in B cells, and prevents apoptosis. Here, we demonstrate a mechanism for activation of the K1 promoter by the Rta transactivator. Electrophoretic mobility shift assay (EMSA) analysis of the K1 promoter demonstrated that purified Rta protein bound to the K1 promoter at three locations, independent of other DNA-binding factors. Transcriptional assays revealed that only two of these Rta DNA-binding sites are functionally significant, and that they could impart Rta responsiveness to a heterologous E4 TATA box promoter. In addition, TATA-binding protein (TBP) bound to a TATA box element located 25 bp upstream of the K1 transcription start site and was also shown to associate with Rta by coimmunoprecipitation analysis. Rta transactivation may therefore be mediated in part through recruitment of TBP to target promoters.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 348, Issue 2, 10 May 2006, Pages 309–327
نویسندگان
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