کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3428045 1594347 2015 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A suppressive effect of Sp1 recruitment to the first leader 5′ splice site region on L4-22K-mediated activation of the adenovirus major late promoter
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
A suppressive effect of Sp1 recruitment to the first leader 5′ splice site region on L4-22K-mediated activation of the adenovirus major late promoter
چکیده انگلیسی
Transcription from the adenovirus major late promoter (MLP) requires binding of late phase-specific factors to the so-called DE element located approximately 100 base pairs downstream of the MLP transcriptional start site. The adenovirus L4-22K protein binds to the DE element and stimulates transcription from the MLP via a DE sequence-dependent mechanism. Here we use a transient expression approach to show that L4-22K binds to an additional site downstream of the MLP start site, the so-called R1 region, which includes the major late first leader 5′ splice site. Binding of L4-22K to R1 has a suppressive effect on MLP transcription. L4-22K binds to the distal part of R1 and stimulates the recruitment of Sp1 and other cellular factors to a site overlapping the first leader 5′ splice site. Binding of Sp1 to the 5′ splice site region had an inhibitory effect on L4-22K-activated MLP transcription.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virus Research - Volume 210, 2 December 2015, Pages 133-140
نویسندگان
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