کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3428175 | 1594352 | 2015 | 5 صفحه PDF | دانلود رایگان |
• Portulaca latent viroid (PoLVd) is a potentially new pospiviroid from Portulaca sp.
• The new viroid shows highest sequence identity with iresine viroid 1 (IrVd-1).
• PoLVd should be considered a new species based on its molecular properties.
• Biological differences between PoLVd and IrVd-1 have not yet been found.
In symptomless plants of portulaca a potential new pospiviroid was characterized. Analysis by both double and return PAGE showed the presence of a circular RNA. RT-PCR and sequencing revealed a genome of 351 nt with properties characteristic of members of the genus Pospiviroid and with highest sequence identity (circa 80%) with iresine viroid 1 (IrVd-1). The circular RNA from portulaca was shown to replicate independently in its original host, thus showing that it is indeed a viroid. Based on its molecular characteristics, it should be considered a new species. However, since no biological differences have yet been found with its closest relative IrVd-1, the viroid from portulaca does not fulfil all criteria for species demarcation of the ICTV.
Journal: Virus Research - Volume 205, 2 July 2015, Pages 22–26