کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3428350 1594362 2015 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Host miR-26a suppresses replication of porcine reproductive and respiratory syndrome virus by upregulating type I interferons
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Host miR-26a suppresses replication of porcine reproductive and respiratory syndrome virus by upregulating type I interferons
چکیده انگلیسی


• Over-expression of the miR-26 family strongly inhibited PRRSV replication in two PRRSV genotypes in a dose-dependent manner.
• Luciferase reporter gene assay showed miR-26a does not target PRRSV genome.
• Over-expression of miR-26a increases the expression of type I IFN and the IFN-stimulated genes MX1 and ISG15 during PRRSV infection.

MicroRNAs (miRNAs) play important roles in viral infections, especially by modulating the expression of cellular factors essential to viral replication or the host innate immune response to infection. To identify host miRNAs important to controlling porcine reproductive and respiratory syndrome virus (PRRSV) infection, we screened 15 miRNAs that were previously implicated in innate immunity or antiviral functions. Over-expression of the miR-26 family strongly inhibited PRRSV replication in vitro, as shown by virus titer assays, Western blotting, and qRT-PCR assays. MiR-26a inhibited the replication of both type 1 and type 2 PRRSV strains. Mutating the seed region of miR-26 restored viral titers. Luciferase reporters showed that miR-26a does not target the PRRSV genome directly but instead affects the expression of type I interferon and the IFN-stimulated genes MX1 and ISG15 during PRRSV infection. These results demonstrate the important role of miR-26a in modulating PRRSV infection and also support the possibility of using host miR-26a to achieve RNAi-mediated antiviral therapeutic strategies.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virus Research - Volume 195, 2 January 2015, Pages 86–94
نویسندگان
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