کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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3429076 | 1228237 | 2011 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Susceptibility and production of a feline endogenous retrovirus (RD-114 virus) in various feline cell lines Susceptibility and production of a feline endogenous retrovirus (RD-114 virus) in various feline cell lines](/preview/png/3429076.png)
RD-114 virus is a replication-competent feline endogenous retrovirus that has been classified as a xenotropic virus. In this study, we examined the expression of the receptors for RD-114 virus in feline cell lines by conducting a pseudotype virus infection assay. Six out of eight feline cell lines were susceptible to the RD-114 pseudotype virus and two cell lines (MCC and FER cells) were resistant. The two resistant cell lines and one cell line (CRFK cells) weakly sensitive to the RD-114 pseudotype virus were found to produce replication-competent RD114-like viruses by the LacZ marker rescue assay and the interference assay. These data strongly suggest that RD-114 virus is polytropic and resistance to RD-114 virus in certain cell lines is due to receptor interference but not polymorphism of the RD-114 receptors. In addition, we determined the amino acid sequences of the envelope region of RD-114-like viruses produced from MCC, FER and CRFK cells. The sequences were identical with the authentic RD-114 virus. Because many feline cell lines are used to manufacture live attenuated vaccines for companion animals, attention should be paid to contamination of the RD-114 virus in vaccines.
Research highlights▶ Six out of eight feline cell lines were susceptible to the RD-114 pseudotype virus. ▶ Two feline cell lines resistant to RD-114 pseudotype produced RD-114-like viruses. ▶ RD-114 virus is polytropic but not xenotropic. ▶ Attention should be paid to contamination of the RD-114 virus in vaccines.
Journal: Virus Research - Volume 155, Issue 1, January 2011, Pages 268–273