کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3429231 1228241 2010 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Open reading frame 60 of the Bombyx mori nucleopolyhedrovirus plays a role in budded virus production
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Open reading frame 60 of the Bombyx mori nucleopolyhedrovirus plays a role in budded virus production
چکیده انگلیسی

Open reading frame 60 (bm60) of the Bombyx mori nucleopolyhedrovirus (BmNPV) is a conserved gene among group I and some group II NPVs. bm60 encodes a late expressed protein that localizes to both the cytoplasm and nucleus of infected cells. This paper describes the characterization of a BmNPV mutant (vbm60-Null) lacking functional bm60. It was observed that the production of budded virus (BV) was reduced by nearly an order of magnitude relative to wt virus in vbm60-Null-infected BmN cells and B. mori larvae. Quantitative real-time PCR assay showed that the viral DNA replication was affected in infected cells due to disruption of bm60. Larval bioassays showed that the speed of kill of vbm60-Null virus was greatly reduced, as it took approximately 28–36 h longer to kill the fifth instar B. mori larvae. These results suggest that BmNPV bm60 is not essential for viral replication, but required for efficient BV production.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virus Research - Volume 151, Issue 2, August 2010, Pages 185–191
نویسندگان
, , , , , , ,