کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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3429566 | 1594394 | 2009 | 5 صفحه PDF | دانلود رایگان |
Hepatitis B virus mutants with in-frame deletions in the central part of the core gene are associated with a severe course of infection in long-term immunosuppressed renal transplant recipients. In this study, yeast two-hybrid system was employed to investigate interaction capabilities of two core mutants with deleted 77–93 and 86–93 amino acids. The same mutant and wild-type (WT) protein pairs which form core-like particles inside bacterial cells were able to interact also in two-hybrid system. To find host proteins possibly involved in enhanced pathogenesis of the mutant variants, a human hepatocyte cDNA library was screened for proteins interacting with the mutant but not with the WT core protein. A human protein of unknown function FLJ20850 interacted specifically with the mutant proteins. An attempt to determine interacting regions revealed that FLJ20850 was unable to interact without significant parts of its C- or N-end, and introduced deletion in the central region conferred interaction capability to the WT core protein.
Journal: Virus Research - Volume 146, Issues 1–2, December 2009, Pages 130–134