کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3429919 1594397 2009 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The Ebola virus ribonucleoprotein complex: A novel VP30–L interaction identified
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
The Ebola virus ribonucleoprotein complex: A novel VP30–L interaction identified
چکیده انگلیسی

The ribonucleoprotein (RNP) complex of Ebola virus (EBOV) is known to be a multiprotein/RNA structure, however, knowledge is rather limited regarding the actual protein–protein interactions involved in its formation. Here we show that singularly expressed VP35 and VP30 are present throughout the cytoplasm, while NP forms prominent cytoplasmic inclusions and L forms smaller perinuclear inclusions. We could demonstrate the existence of NP–VP35, NP–VP30 and VP35–L interactions, similar to those described for Marburg virus (MARV) based on the redistribution of protein partners into NP and L inclusion bodies. Significantly, a novel VP30–L interaction was also identified and found to form as part of an NP–VP30–L bridge structure, similar to that formed by VP35. The identification of these interactions allows a preliminary model of the EBOV RNP complex structure to be proposed, and may provide insight into filovirus transcriptional regulation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virus Research - Volume 140, Issues 1–2, March 2009, Pages 8–14
نویسندگان
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