کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3430331 1228272 2008 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Mutational events during the primary propagation and consecutive passages of hepatitis E virus strain JE03-1760F in cell culture
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Mutational events during the primary propagation and consecutive passages of hepatitis E virus strain JE03-1760F in cell culture
چکیده انگلیسی

We recently developed a cell culture system for hepatitis E virus (HEV) in PLC/PRF/5 cells, using a genotype 3 HEV (JE03-1760F strain). Thirteen generations of consecutive passages of culture supernatant were successfully carried out in PLC/PRF/5 cells, with the highest HEV load reaching 108 copies/ml in the culture medium. Based on continuous release of progenies into culture medium, 50% tissue culture infectivity doses were estimated to be 2.0 × 103 copies for wild-type JE03-1760F and 1.4 × 102 copies for p13 (progeny in the thirteenth passage). Earlier appearance and greater increase in the yield of progenies in the culture supernatant were evident in p13 compared with wild-type. The cell culture-produced variants in primary propagation (p0) and consecutive passages (p5 [fifth passage], p10 [tenth], and p13) differed from the wild-type virus by 1, 9, 18, and 19 nucleotides (nt), respectively, over the entire genome of 7226 nt, excluding the poly(A) tail. Three of five non-synonymous mutations in p13 were shared by a variant (fifth passage) in another series of passages of JE03-1760F. These results suggest that adaptation of HEV variants to growth in vitro is associated with a limited number of mutations similar to hepatitis A virus.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virus Research - Volume 137, Issue 1, October 2008, Pages 86–96
نویسندگان
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