کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3430471 1594399 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Mitochondrial plasmids of sugar beet amplified via rolling circle method detected during curtovirus screening
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Mitochondrial plasmids of sugar beet amplified via rolling circle method detected during curtovirus screening
چکیده انگلیسی
Crops of sugar beet have been considerably impaired by infection with Beet curly top virus (BCTV) during the past decades. Quick and reliable diagnostic techniques are therefore desirable to detect this circular single-stranded DNA-containing geminivirus. Techniques combining either tissue printing or blot hybridization, or rolling circle amplification (RCA) and restriction fragment length polymorphism (RFLP) were compared. Although they easily detected BCTV with certainty, both exhibited apparent false positive results which have been scrutinized in closer detail. Uninfected control plants revealed unspecific signals due to probe attachment on tissue blots, and dominant fragment patterns upon RCA/RFLP which did not hybridize with BCTV-specific probes. Cloning and sequencing of these DNA fragments showed that they were amplified from mitochondrial plasmids. Examination of their genome structure revealed no relationship with geminiviruses or their satellites.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virus Research - Volume 136, Issues 1–2, September 2008, Pages 124-129
نویسندگان
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