کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3430839 1228285 2007 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Establishment of an immortal turkey turbinate cell line suitable for avian metapneumovirus propagation
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Establishment of an immortal turkey turbinate cell line suitable for avian metapneumovirus propagation
چکیده انگلیسی

Until recently, there has not been a homologous avian cellular substrate which could continuously produce high titer avian metapneumovirus (AMPV); development of such a cell line should provide an excellent model system for studying AMPV infection. We have established a non-tumorigenic immortal turkey turbinate cell line (TT-1) to propagate sufficiently high AMPV titers. Currently, immortal TT-1 cells are growing continuously at 1.2–1.4 population doublings per day and are at passage 160. Kinetic analysis suggests that AMPV can infect and replicate more rapidly in TT-1 compared to Vero cells, although both cell types undergo apoptosis upon infection. The non-tumorigenic, reverse transcriptase negative TT-1 cell line can serve as an excellent homologous cellular substrate for virus propagation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virus Research - Volume 127, Issue 1, July 2007, Pages 106–115
نویسندگان
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