کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3430861 1228286 2007 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
In vitro cell fusion between CD4+ and HIV-1 Env+ T cells generates a diversity of syncytia varying in total number, size and cellular content
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
In vitro cell fusion between CD4+ and HIV-1 Env+ T cells generates a diversity of syncytia varying in total number, size and cellular content
چکیده انگلیسی

Syncytia formation in HIV infections is driven by the virus fusion-active molecules (Env) interacting with membrane components of hosts cells. HIV-syncytia are usually interpreted as pathogenic entities and although they may potentially vary in size, numbers and types of constituent cells, little is known about the extent and significance of their diversity. Here, we describe numerically the cell population dynamics and the diversity of syncytia produced in the in vitro cell-fusion between two Jurkat T cell lines, one CD4+ and the other Env+. Cell-fusion partners were differentially stained with the lipophilic DiI and DiO, or with the cytoplasmic CMFDA and CMTMR tracers and syncytia showing double fluorescence were counted in a flow cytometer. The total number of syncytia formed, their size, cellular complexity and ratio of CD4+/Env+ cells recruited, varied significantly in relation with time of reaction and initial proportions of fusion partners. The considerable structural diversity of syncytia formed, in so limited an in vitro cell fusion reaction, suggests that a greater heterogeneity may be formed in the natural course of disease. Identification of the main determinants of syncytia diversity allows for a detailed study of the relation between the syncytia structure and function.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virus Research - Volume 123, Issue 2, February 2007, Pages 138–146
نویسندگان
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