کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3431180 1594406 2006 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Synthesis of minus-strand copies of a viral transgene during viral infections of transgenic plants
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Synthesis of minus-strand copies of a viral transgene during viral infections of transgenic plants
چکیده انگلیسی

Viral transgenes designed to provide resistance to specific plant viruses frequently consist of the coat protein gene and a contiguous 3′ untranslated region (3′UTR) of viral origin. In many RNA viruses the viral 3′UTR establishes a recognition and initiation site for viral RNA replication. Thus the transgenic transcript may contain a functional virus replication site. Experiments were designed to determine if a challenging virus would recognize this replication site on a nuclear derived transcript and synthesize the complementary RNA. These data demonstrate that upon infection by a virus that recognizes the viral replication site, a full-length complement of the transgenic transcript is produced. In these experiments the replication complex of Brome Mosaic bromovirus recognized the transgenic transcript derived from a Cowpea Chlorotic Mottle bromovirus transgene. The resulting RNA may contribute to RNA recombination events.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virus Research - Volume 122, Issues 1–2, December 2006, Pages 171–174
نویسندگان
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