کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3431257 1228296 2006 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Studies on the activity of a bidirectional promoter of Mungbean yellow mosaic India virus by agroinfiltration
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Studies on the activity of a bidirectional promoter of Mungbean yellow mosaic India virus by agroinfiltration
چکیده انگلیسی

The AV promoter expressing AV1 and AV2 genes and AC1 promoter expressing AC1 gene are present in opposite orientation in the intergenic region of Mungbean yellow mosaic India virus (MYMIV). Transient Agrobacterium-mediated delivery of putative promoter constructs into Nicotiana benthamiana and different legumes, followed by reporter gene (β-d-glucuronidase, GUS) assay, identified the promoter region of both AC1 and AV genes that is necessary for transcriptional initiation. Transcription activator protein-independent activity of AV promoter and differential regulation of AC1 promoter are unique to MYMIV. The AV promoter is a composite core promoter having both TATA box and Initiator elements (TATA+Inr+). Many transcription factor binding sites were identified in the upstream promoter sequences of both virion and complementary sense genes, which might be used in the transcription regulation studies of the host plant as well as the virus.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virus Research - Volume 119, Issue 2, August 2006, Pages 154–162
نویسندگان
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