کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
34380 45020 2014 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Multi-stage laccase extraction and separation using aqueous two-phase systems: Experiment and model
ترجمه فارسی عنوان
استخراج و جداسازی لاکتوز چند مرحله ای با استفاده از سیستم های دو فازی آبی: آزمایش و مدل
کلمات کلیدی
استخراج دو مرحله ای آبی، لاکاس، آزمایش های چند مرحله ای، مدل سازی تعادل مرحله ای، افزایش مقیاس
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی


• PEG3000-PO4 ATPS used for laccase purification and separation.
• Multi-stage ATPE with two modes: selective separation or enzyme purification.
• Process model for ATPE of enzymes.

This work presents results of experimental and model investigation of continuous multi-stage enzyme extraction using aqueous two-phase systems for the first time. The aqueous two-phase system comprised polyethylene glycol 3000 and phosphate with additional sodium chloride buffered to pH 7. Two different laccases served as model enzymes. One of the laccases was directly taken from fungal culture supernatant, while the other laccase was solubilized lyophilisate. The modeling is based on an equilibrium stage approach. Equilibrium data were taken from single-stage experiments and approximated by different correlation equations. The model describes densities, phase equilibrium, enzyme activity partitioning between the phases. Moreover it allows to consider activity changes due to the aqueous two-phase system. Eight multi-stage mixer-settler experiments under varying operation conditions were performed to validate the proposed model; whereas the total throughput of all multi-stage extraction experiments was about 350 g h−1. The average relative deviation of modeled activities from experimentally measured activities was 23%. Therefore, the model is able to calculate the behavior of the phases as well as the partitioning of the two enzymes between the two phases for a multi-stage process based on single-stage data.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Process Biochemistry - Volume 49, Issue 6, June 2014, Pages 1020–1031
نویسندگان
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