کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
35194 | 45080 | 2010 | 4 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Extraction and three-phase partitioning behavior of proteases from papaya peels Extraction and three-phase partitioning behavior of proteases from papaya peels](/preview/png/35194.png)
Dried papaya peels exhibited superior proteolytic activity to the fresh peels. An extraction with phosphate buffer pH 7.0 greatly maintained proteolytic activity when compared to water. SDS-PAGE verified that the extracted dried papaya peels held a wide range of proteins. To optimize the three-phase partitioning (TPP) for isolating the papaya peel proteases required a ratio of crude extract to t-butanol, the (NH4)2SO4 concentration and the TPP cycles. The ratio of the crude extract to t-butanol of 1.0:0.5 with the presence of 20% (NH4)2SO4 resulted in the highest proteolytic recovery at 253.5%, and 15.8-fold of purification in the bottom phase. The TPP was then optimized by adding up to 55% (NH4)2SO4 to the bottom phase of the first step. A purification of 10.1-fold with about 89.4% recovery was obtained. This study showed the TPP can be effectively employed for the extraction of proteases from papaya peels.
Journal: Process Biochemistry - Volume 45, Issue 7, July 2010, Pages 1172–1175