کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
35465 45091 2008 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Production and characterization of a novel antimicrobial peptide HKABF by Pichia pastoris
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Production and characterization of a novel antimicrobial peptide HKABF by Pichia pastoris
چکیده انگلیسی

HKABF, Hippocampus kuda antibacterial factor, is a putative antimicrobial peptide identified from the cDNA library of the brooding pouch of H. kuda Bleeker. The putative mature peptide, fused with a 6His-tag, an anionic peptide (SAPLNPDNID) and to an enterokinase cleavage site was expressed in Pichia pastoris. The mature HKABF peptide, released by enterokinase cleavage, showed antimicrobial activity against Staphylococcus aureus and Staphylococcus saprophyticus at low concentrations (0.5–2.5 μg/ml). The recombinant mature peptide was heat-stable and had low hemolytic activity. Circular dichroism (CD) spectroscopy data indicates its classification as a CSαβ-type antimicrobial peptide. This study serves as an impetus for the production and further research of this antimicrobial peptide.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Process Biochemistry - Volume 43, Issue 10, October 2008, Pages 1124–1131
نویسندگان
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