کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
35848 45110 2008 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Evaluation of modified chitosan as matrix for hydrophobic interaction chromatography
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Evaluation of modified chitosan as matrix for hydrophobic interaction chromatography
چکیده انگلیسی

Chitosan beads were modified with glutaraldehyde and modified chitosan was investigated as matrix for hydrophobic interaction chromatography. The influence of temperature, type of salt and its ionic strength on the adsorption of α-galactosidase was studied. α-Galactosidase was found to bind in presence of high concentration of ammonium sulphate (3 M, w/v) and 90% of the bound enzyme was eluted with decreasing salt concentration in presence of 10% ethylene glycol. Attempt was made to purify α-galactosidase from modified chitosan, α-galactosidase showed 1.7-fold purification with 43.96% recovery of enzyme activity. The SDS–PAGE analysis of enzyme showed considerable purification and its molecular weight was found to be 63–64 kDa. Unlike other chromatographic matrices, the prepared chitosan beads were used five times. The results showed that purification and recovery of the enzyme did not change even when column size was increased.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Process Biochemistry - Volume 43, Issue 6, June 2008, Pages 647–653
نویسندگان
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