کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
36443 45133 2005 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Production of pyruvate from lactate using recombinant Pichia pastoris cells as catalyst
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Production of pyruvate from lactate using recombinant Pichia pastoris cells as catalyst
چکیده انگلیسی

Because of the synergistic presence of enzyme systems, whole-cell biocatalysts are an advantageous means of oxidizing α-hydroxy to α-keto carboxylic acids. The effect of different parameters on conversion were determined when whole cells converted l-lactate to pyruvate. The biocatalyst was a double recombinant Pichia pastoris containing a glycolate oxidase and catalase enzyme system. A marked increase in conversion occurred with oxygen compared to air. This indicated that the oxygen concentration was an important factor in the rate-limiting step. Lactate concentrations above 0.5 M showed substrate inhibition. Although temperature played an important role in enzyme stability, whole-cell tranformants were much more stable over time than soluble enzymes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Process Biochemistry - Volume 40, Issue 8, July 2005, Pages 2597–2601
نویسندگان
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