کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
36556 | 45136 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Hydrolysis of starch by amylase from Bacillus sp. KCA102: a statistical approach
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
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چکیده انگلیسی
Bacillus sp. KCA102 capable of producing thermostable amylase in a short fermentation time (∼18–20 h) has been isolated from a soil sample. The performance of amylase during hydrolysis of starch has been studied using response surface methodology. The parameters under study have been categorized into two, viz., physical parameters (pH, temperature and time) and chemical parameters (amounts of substrate and enzyme). The optimal conditions of pH, temperature and time at which maximum amount of glucose formed were found to be 7.1, 57.5 °C and 25 min, respectively. The optimal amounts of substrate and enzyme for maximum hydrolysis were found to be 0.5% starch and 94 mU/ml of amylase, respectively.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Process Biochemistry - Volume 40, Issue 7, June 2005, Pages 2499–2507
Journal: Process Biochemistry - Volume 40, Issue 7, June 2005, Pages 2499–2507
نویسندگان
Manoj Agrawal, S. Pradeep, K. Chandraraj, Sathyanarayana N. Gummadi,