کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3748 185 2011 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Comparison of substance supply in static and perfusion cultures based on mass transport phenomena
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Comparison of substance supply in static and perfusion cultures based on mass transport phenomena
چکیده انگلیسی

Mass transport phenomena in cell culture can be formulated by using classical reaction-diffusion equations; however, in practice, it is difficult to solve these equations analytically. Here, we used computer simulation to solve these equations, and compared the time-dependent concentration profile of substances in conventional static culture with that in perfusion culture. The simulated glucose consumption in static culture agreed with that of actual culture conditions used in a general cell culture experiment. The simulation of perfusion culture revealed that the geometry of the chamber and its operating parameters are critical to obtaining a sufficient supply of substances. We also found that the previously reported perfusion chambers are well designed and operate under adequate conditions. Our kinetic model of time-dependent concentration profiles for general substances based on mass transport phenomena could, therefore, be used for the optimal design of a microfluidic perfusion culture chip.


► Mathematical modeling was used to assess substance supply in cell cultures.
► Simulation of static culture conditions resembles actual culture conditions.
► Simulation was used to evaluate previously reported perfusion chambers.
► The mathematical model could be use to design a microfluidic cell culture chip.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Engineering Journal - Volume 57, 15 November 2011, Pages 69–74
نویسندگان
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