کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3817631 | 1597729 | 2016 | 7 صفحه PDF | دانلود رایگان |
• Cold atmospheric plasma strongly inhibited C. albicans growth and ergosterol biosynthesis.
• Cold atmospheric plasma inhibited biofilm formation in C. albicans in the range of 43–57%.
• Cold atmospheric plasma suppressed phospholipase and proteinase activity in C. albicans.
• Cold atmospheric plasma is a potential candidate to treat Candida-related superficial infections.
BackgroundThe pathogenic yeast Candida albicans is the most common opportunistic fungal pathogen that is responsible for a wide array of infections in susceptible individuals. Despite recent progress in developing novel antifungal drugs which combat Candida-related disorders, this fungus is still a major cause of life-threatening infections all over the world. In the present study, the effect of cold atmospheric plasma (CAP) was evaluated on the growth of C. albicans with special attention to the ability of the CAP-treated fungus for biofilm formation, ergosterol biosynthesis and phospholipase and proteinase secretory production.MethodsC. albicans cell suspensions were irradiated over time-scales ranging of 90, 120, 150 and 180 s under cold atmospheric plasma contained He/O2 (2%). Treated and untreated yeast cells were analyzed for the growth, biofilm formation, ergosterol content, and activities of phospholipase and proteinase.ResultsOur results showed that CAP remarkably suppressed the growth of C. albicans by 31–82% at the given times. Likewise, CAP strongly inhibited the ergosterol biosynthesis by the fungus in the range of 40–91%, biofilm formation by 43–57% and the activities of phospholipase and proteinase enzymes by 4–45%, dose-dependently.ConclusionCAP strongly inhibits the growth and virulence factors of C. albicans and thus, it could be a potential candidate to treat Candida-related superficial and cutaneous infections in practice.
Journal: Photodiagnosis and Photodynamic Therapy - Volume 13, March 2016, Pages 66–72