کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3840582 1247924 2011 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Gefitinib attenuates transforming growth factor-β1–activated mitogen-activated protein kinases and mitogenesis in NRK-49F cells
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی پزشکی و دندانپزشکی (عمومی)
پیش نمایش صفحه اول مقاله
Gefitinib attenuates transforming growth factor-β1–activated mitogen-activated protein kinases and mitogenesis in NRK-49F cells
چکیده انگلیسی

Transforming growth factor-β (TGF-β), TGF-β receptor (TGF-βR), and epidermal growth factor receptor (EGFR) are important in the pathogenesis of kidney fibrosis, a result of renal fibroblast activation. The EGFR kinase inhibitor gefitinib attenuates glomerular fibrosis in hypertensive rats whereas dominant-negative EGFR attenuates interstitial fibrosis in mouse with acute renal ischemia. Thus, we studied the effects and molecular mechanisms of gefitinib in TGF-β1–induced mitogenesis and collagen production in normal rat kidney interstitial fibroblast (NRK-49F) cells. We found that TGF-β1 increased cell mitogenesis. TGF-β1 also time-dependently increased cyclin D1 protein expression. TGF-β1 rapidly transactivated EGFR. SB431542 (a type I TGF-βR kinase inhibitor) and SB203580 (a p38 kinase inhibitor) attenuated TGF-β1–induced phosphorylation of Smad2/3 protein. SB431542 and gefitinib attenuated TGF-β1–induced phosphorylation of ERK1/2 and p38 kinase. SB431542 and gefitinib also attenuated TGF-β1–induced cyclin D1 protein expression. Moreover, SB431542, gefitinib, PD98059 (an ERK1/2 inhibitor), and SB203580 attenuated TGF-β1–induced cell mitogenesis. Finally, SB431542 and gefitinib attenuated TGF-β1–induced collagen production. We concluded that gefitinib attenuates TGF-β1–induced cell mitogenesis via the EGFR-ERK1/2/p38 kinase pathway in NRK-49F cells. Moreover, gefitinib attenuates TGF-β1–induced cyclin D1 protein expression and collagen production. Thus, gefitinib attenuates TGF-β1–induced mitogenesis and collagen production in vitro.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Translational Research - Volume 158, Issue 4, October 2011, Pages 214–224
نویسندگان
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