کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3840736 1247933 2011 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Long-term renal changes in the liver-specific glucokinase knockout mouse: implications for renal disease in maturity-onset diabetes of the young 2
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی پزشکی و دندانپزشکی (عمومی)
پیش نمایش صفحه اول مقاله
Long-term renal changes in the liver-specific glucokinase knockout mouse: implications for renal disease in maturity-onset diabetes of the young 2
چکیده انگلیسی

To investigate the functional and structural renal changes in a long-term liver-specific glucokinase (gck) knockout mouse, a model was developed of maturity-onset diabetes of the young (MODY2). Hemizygous gck knockout mice, gckw/– groups, were compared at 6, 10, and 14 months with their age-matched normal littermates, gckw/w groups. To examine changes, we compared body weight, fasting blood glucose, serum insulin, and creatinine levels, as well as 24-h urine samples that were collected for urine volume and protein analysis between the 2 groups. Renal tissues were collected and stained with hemotoxylin-eosin and periodic-acid Schiff for light microscopic observation. The expression of renal transforming growth factor β1 (TGF-β1) was determined by Western blot. Our results show that fasting blood glucose levels were significantly higher in gckw/− mice compared with gckw/w mice (P < 0.01) for all age groups. Compared with age-matched gckw/w mice, 10-month old gckw/– mice have significantly elevated body weights (P < 0.01) and protein contents (P < 0.001). A gradual increase in mesangial matrix and a thickening of the glomerular basement membrane was observed in gckw/− mice at 10 and 14 months. The levels of renal TGF-β1 expression are increasing in both gckw/− and gckw/w mice. Our results indicate that renal changes occur in the liver-specific gck knockout mouse model of MODY2 and suggest that TGF-β1 may play a key role in pathogenesis of these renal changes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Translational Research - Volume 157, Issue 3, March 2011, Pages 111–116
نویسندگان
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