کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3999854 1601828 2012 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Overexpression of cyclin-dependent kinase-associated protein phosphatase enhances cell proliferation in renal cancer cells
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی تومور شناسی
پیش نمایش صفحه اول مقاله
Overexpression of cyclin-dependent kinase-associated protein phosphatase enhances cell proliferation in renal cancer cells
چکیده انگلیسی

ObjectiveThe aim of this study was to understand the role of cyclin-dependent kinase-associated protein phosphatase (KAP) in renal cancer cell growth.Materials and methodsRenal cell carcinoma (RCC) tissues from 58 patients receiving surgical resection were included for immunohistochemistry analysis. Additionally, human embryonic kidney (HEK293) cells overexpressing KAP were established for tumorigenicity experiments.ResultsClinicopathologic analysis indicated that poorly differentiated RCCs with a higher histological grade (grade 3/4) were associated with a higher proportion of KAP-positive cells (P < 0.001) as well as cytoplasmic expression of KAP (P < 0.05). HEK293 cells overexpressing KAP had a higher growth rate, greater resistance to TNF-α mediated increment of caspase 3 activity, a shorter cell cycle time, and greater ability of cell invasion. Tumorigenicity experiments showed that KAP-overexpressing cells generated significantly larger xenograft tumors in nude mice compared with mock controls (P = 0.032).ConclusionsKAP expression was associated with poorly differentiated RCCs and overexpression of KAP in renal cells enhanced cell proliferation, resistance to apoptosis, invasive ability, and xenograft tumor formation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Urologic Oncology: Seminars and Original Investigations - Volume 30, Issue 6, November–December 2012, Pages 871–878
نویسندگان
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