کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4000071 1259365 2013 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Urine Cell-Free DNA integrity as a marker for early bladder cancer diagnosis: Preliminary data
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی تومور شناسی
پیش نمایش صفحه اول مقاله
Urine Cell-Free DNA integrity as a marker for early bladder cancer diagnosis: Preliminary data
چکیده انگلیسی

ObjectivesUrine cell-free (UCF) DNA has recently been proposed as a potential marker for early bladder cancer diagnosis. It is known that normal apoptotic cells produce highly fragmented DNA while cancer cells release longer DNA. Therefore, we verified the potential role of UCF DNA integrity in early bladder cancer diagnosis.Materials and methodsUCF DNA was isolated from 51 bladder cancer patients, 46 symptomatic patients, and 32 healthy volunteers. To verify UCF DNA integrity, sequences longer than 250 bp, c-Myc, BCAS1, and HER2, were quantified by real time PCR.ResultsAt the best cutoff value of 0.1 ng/μl, UCF DNA integrity analysis showed a sensitivity of 0.73 (95% CI 0.61–0.85), and a specificity of 0.84 (95% CI 0.71–0.97) in healthy individuals and 0.83 (95% CI 0.72–0.94) in symptomatic patients. Receiver operating characteristic (ROC) curve analysis revealed an area under the curve of 0.834 (95% CI 0.739–0.930) for healthy individuals and 0.796 (95% CI 0.707–0.885) for symptomatic patients.ConclusionsThese preliminary data suggest that UCF DNA integrity is a potentially good marker for early noninvasive diagnosis of bladder cancer. Its diagnostic performance does not seem to vary significantly, even in an “at risk” population of symptomatic individuals.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Urologic Oncology: Seminars and Original Investigations - Volume 31, Issue 8, November 2013, Pages 1744–1750
نویسندگان
, , , , , , , , , , , ,