کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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4011734 | 1261160 | 2011 | 11 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Retinal degeneration in two lines of transgenic S334ter rats Retinal degeneration in two lines of transgenic S334ter rats](/preview/png/4011734.png)
Aim of this study was to examine synaptic connectivity changes in the retina and the location and rate of apoptosis in transgenic S334ter line-3 and line-5 rats with photoreceptor degeneration. Heterozygous S334ter-line-3 and line-5 at P11-13, P30, P60, P90 and several control non-dystrophic rats (Long Evans and Sprague–Dawley) at P60, were studied anatomically by immunohistochemistry for various cell and synaptic markers, and by PNA and TUNEL label.- S334ter line-3 exhibited the fastest rate of degeneration with an early loss of photoreceptors, with 1–2 layers remaining at P30, and only cones left at P60. Line-5 had 4–5 layers left at P30, and very few rods left at P60-90. In both lines, horizontal cell processes (including dendrites and axon) were diminished at P11-13, showing gaps in the outer plexiform layer (OPL) at P60, and at P90, almost no terminal tips could be seen. Bipolar cells showed a retraction of their dendrites forming clusters along the OPL. Synaptic terminals of A-II amacrine cells in the IPL lost most of their parvalbumin-immunoreactivity. The apoptosis rate was different in both lines. Line-3 rats showed many photoreceptors affected at P11, occupying the innermost part of the outer nuclear layer. Line-5 showed a lower number of apoptotic cells within the same location at P13. In summary, the S334ter line-3 rat has a faster progression of degeneration than line-5. The horizontal and bipolar terminals are already affected at P11-P13 in both models. Apoptosis is related to the mutated rhodopsin transgene; the first photoreceptor cells affected are those close to the OPL.
Figure optionsDownload high-quality image (90 K)Download as PowerPoint slideResearch highlights
► S334ter line-3 rats have a faster progression of degeneration than line-5.
► Rod degeneration affects synaptic connectivity of horizontal and bipolar cells.
► Horizontal and bipolar terminals are already affected at P11–P13 in both models.
► Apoptosis affects first the photoreceptors close to the OPL.
Journal: Experimental Eye Research - Volume 92, Issue 3, March 2011, Pages 227–237